Previously, we demonstrated that nucleotides released upon mechanical injury to corneal epithelium activate purinergic (P2) receptors resulting in mobilization of a Ca(2+) wave.However, the tissue is extensively innervated and communication between epithelium and neurons is critical and not well understood.Therefore, we developed a co-culture of pr
Quantitation of non-derivatized free amino acids for detecting inborn errors of metabolism by incorporating mixed-mode chromatography with tandem mass spectrometry
Introduction: Amino acids are critical biomarkers for many inborn errors of metabolism, but Jeans amino acid analysis is challenging due to the range of chemical properties inherent in these small molecules.Techniques are available for amino acid analysis, but they can suffer from long run times, laborious derivatization, and/or poor resolution of
Detecting Winter Cover Crops and Crop Residues in the Midwest US Using Machine Learning Classification of Thermal and Optical Imagery
Cover crops are an increasingly popular practice to improve agroecosystem resilience to climate change, pests, and other stressors.Despite their importance for climate mitigation and soil health, there remains an urgent need for methods that link winter cover crops with regional-scale climate mitigation and adaptation potential.Remote sensing is id
Changes in the pitch-accent system of Gernika Basque
The pitch-accent system in Northern Bizkaian Basque (NBB) is characterized for making a pitch distinction between lexical accented/unaccented words (i.e.singular vs.plural contrast) and for lacking a durational correlate of accentual prominence.The present study examines whether such system is maintained jeff rosenstock buffalo in Gernika Basque, a
Lessons from the Use of Ranked Choice Voting in American Presidential Primaries
Grounded in experience in 2020, both major political parties have reasons to expand use of ranked choice voting (RCV) in their 2024 presidential primaries.RCV may offer a ‘win-win’ solution benefiting both the parties and their voters.RCV would build on both the pre-1968 American tradition of parties determining a coalitional presidential nomin